Journal: The Journal of Neuroscience
Article Title: Selective Elimination of Corticogeniculate Feedback Abolishes the Electroencephalogram Dependence of Primary Visual Cortical Receptive Fields and Reduces Their Spatial Specificity
doi: 10.1523/JNEUROSCI.23-18-07021.2003
Figure Lengend Snippet: Highly selective elimination of corticogeniculate neurons in areas 17-18 of experimental cortex. a, Gyrus lateralis of an experimental cat 14 d after induction of apoptosis. The red arrow indicates the center and direction of photoactivation by incident light (1.9 mm diameter region of photoactivation); L, lateral; V, ventral; WM, white matter. b, c, Merged overlay of images for the rhodamine fluorochrome carried by the targeting nanospheres (red) and immunocytochemical labeling for the mature neuronal marker NeuN with Cy2 (green). b, High magnification image of the blue-boxed region in a, demonstrating the highly efficient elimination of corticogeniculate projection neurons in the experimentally targeted region. c, High magnification image of the red-boxed region in a, demonstrating the normal appearance of corticogeniculate neurons in control primary visual cortex. d-f, Quantification of corticogeniculate neuron loss by relative cell density profiles. Regions denoted by gray and white shading represent assignment to specific cortical layers (noted above the abscissa). d, Density profile in regions of corticogeniculate neuron apoptosis at the tip of the gyrus and in the homologous regions in the control hemisphere (see insets above). e, Control region at the tip of the gyrus 3-6 mm anterior to the affected region, within the same experimental hemisphere (see inset). f, Control regions deep in the medial or lateral banks of the experimental hemisphere. Note that layer 5A is relatively thicker than layers 5B-6 in these deep regions compared with that at the tip of the gyrus, as indicated by three sampling windows and thicker area of gray shading. Error bars in d-f indicate SDs from the means.
Article Snippet: Sections from the regions of targeted neuronal apoptosis and the homologous regions of the control hemispheres were processed for immunocytochemistry using primary antibodies directed against the mature neuronal marker NeuN (Chemicon, Temecula, CA).
Techniques: Labeling, Marker, Sampling
